The MultiAmplicon R-package

The MultiAmplicon-package allows the matching and removal of primer sequences from sequencing reads. Amplicons, here defined as the sequences amplified by particular combination of forward and reverse primer, can then be further processed. The package guiedes downstream analysis of multiple amplicons in a bioconductor workflow wrapped around the package dada2 and helps to export to phyloseq for further analysis.

Installation

require(devtools)
devtools::install_github("derele/MultiAmplicon")

Documentation

Documentation, including the reference manual is available online.

Tutorials cover:

• A a typical workflow quickly executed on data available within the package

• A a typical workflow quickly executed on a real world dataset