This repository provides a minimal pipeline to process single-molecule FRET (.tracks/.h5) data[1] and fit a
three-state kinetic model (Open ↔ Intermediate ↔ Closed) with state-dependent bleaching.
[1] Hugel, T. (2025). Datasets for “Single-molecule FRET and tracking of transfected biomolecules in living cells” [Data set]. Biophysical Journal, 125(March 3), 1–9. Zenodo. https://doi.org/10.5281/zenodo.17559063
[2] Schrangl, L., Göhring, J., Kellner, F., Huppa, J. B., & Schütz, G. J. (2021). Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes. Journal of Visualized Experiments, 177, e63124. https://doi.org/10.3791/63124
[3] Anandamurugan, A., Eidloth, A., Frank, V., Wortmann, P., Schrangl, L., Lan, C., Schütz, G. J., & Hugel, T. ( 2025). Single-molecule FRET and tracking of transfected biomolecules in living cells. Biophysical Journal, S0006-3495(25)00604-6. Advance online publication. https://doi.org/10.1016/j.bpj.2025.09.024