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Analyze Particles ImageJ

This script runs particle analysis on nd2 files in one directory.

  • open nd2 file with split channels
  • for TRITC channel:
    • maximum intensity projection
    • AutoThreshold (Method: Default, "ignore black")
    • Watershed
    • analyze particles
  • saves:
    • maximum intensity projection images with particle outlines
    • drawings of the outlines
    • results csv for every single image
    • at the end: summary csv for all images

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