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Biolens

AI-Powered Gene Expression Analysis Platform

Python FastAPI PostgreSQL License

An end-to-end bioinformatics platform for querying, analyzing, and interpreting TCGA breast cancer gene expression data. Combines a PostgreSQL relational database, FastAPI REST API, Claude-powered LLM agent, and an interactive Streamlist UI.

** Live API Docs** (if deployed)


Features

Feature Description
PostgreSQL database 15K genes × 1,218 TCGA-BRCA samples
FastAPI REST API Expression queries, DE analysis, pathway enrichment
Claude LLM Natural language biological interpretation
LangChain agent Autonomous multi-step analysis workflows
ChromaDB search Semantic gene search by biological function
ML classifier GradientBoosting PAM50 subtype prediction (XX% accuracy)
AWS deployment EC2 + S3 + Docker
Streamlit UI Interactive visual interface

Tech Stack

Python · FastAPI · PostgreSQL · LangChain · Anthropic Claude API · ChromaDB · scikit-learn · AWS (EC2/S3) · Docker · Streamlit · Plotly

Quick Start

git clone https://github.com/SanchithaK/biolens.git
cd biolens
cp .env.example .env        # Add your API keys
sudo service postgresql start
source venv/bin/activate
uvicorn backend.main:app --reload --port 8000 &
streamlit run app.py

Screenshots

[Add screenshots here]

API Endpoints

Endpoint Method Description
/expression/{gene} GET Expression values by sample/stage
/expression/samples GET List samples with filters
/expression/search/semantic GET Vector similarity gene search
/analysis/differential POST DE analysis between groups
/analysis/classify/{sample_id} GET ML cancer subtype prediction
/chat/ask POST Claude AI interpretation

Data

Uses publicly available TCGA-BRCA RNA-seq data (1,218 samples). Download from UCSC Xena.

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