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FAβ-gal is a method that joins the simplicity, fast execution, low cost and versatility of the classical SA-β-gal assay with the quantification and sensitivity of fluorescence assays. It exploits the far-red fluorescence of X-gal product (Levitsky et al., 2013) coupled with a count of cell nuclei powered by BiaPy to quantify in a semiautomatic and unbiased manner SA-β-gal activity. To read the full method go to FAβ-gal publication