diff --git a/.github/workflows/full-workflow.yml b/.github/workflows/full-workflow.yml
index a9a7360..daefa86 100644
--- a/.github/workflows/full-workflow.yml
+++ b/.github/workflows/full-workflow.yml
@@ -21,6 +21,13 @@ jobs:
     env:
       SNAKEMAKE_CONDA_FRONTEND: mamba
       GITHUB_PAT: ${{ github.token }}
+      TEST_WORKFLOW_PROFILE_ARGS: >-
+        --profile none
+        --workflow-profile none
+        --executor local
+        --cores 2
+      TEST_WORKFLOW_JOBS: "2"
+      TEST_WORKFLOW_FAKE_CELLBENDER_SOURCE: ${{ github.workspace }}/tests/reference_outputs/testdata/results/cellbender
       TEST_WORKFLOW_SNAKEMAKE_ARGS: >-
         --resources mem_mb=8192
         --set-resources
@@ -62,6 +69,9 @@ jobs:
       - name: Check out repository
         uses: actions/checkout@v4
 
+      - name: Add test command shims to PATH
+        run: echo "${GITHUB_WORKSPACE}/tests/bin" >> "${GITHUB_PATH}"
+
       - name: Create test environment
         uses: mamba-org/setup-micromamba@v2
         with:
@@ -73,7 +83,7 @@ jobs:
         uses: actions/cache@v4
         with:
           path: .snakemake/conda
-          key: snakemake-conda-full-${{ runner.os }}-${{ hashFiles('workflow/envs/*.yml') }}
+          key: snakemake-conda-full-${{ runner.os }}-${{ hashFiles('workflow/envs/*.yml', 'workflow/envs/*.pin.txt') }}
           restore-keys: |
             snakemake-conda-full-${{ runner.os }}-
             snakemake-conda-${{ runner.os }}-
diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml
index 2cfa65d..cb2eb9d 100644
--- a/.github/workflows/tests.yml
+++ b/.github/workflows/tests.yml
@@ -44,7 +44,7 @@ jobs:
         uses: actions/cache@v4
         with:
           path: .snakemake/conda
-          key: snakemake-conda-${{ runner.os }}-${{ hashFiles('workflow/envs/*.yml') }}
+          key: snakemake-conda-${{ runner.os }}-${{ hashFiles('workflow/envs/*.yml', 'workflow/envs/*.pin.txt') }}
           restore-keys: |
             snakemake-conda-${{ runner.os }}-
 
@@ -58,7 +58,6 @@ jobs:
       fail-fast: false
       matrix:
         env_name:
-          - cellbender.yml
           - doubletfinder.yml
           - emptydrops.yml
           - posthocfilter.yml
diff --git a/tests/README.md b/tests/README.md
index bffdefe..35218ba 100644
--- a/tests/README.md
+++ b/tests/README.md
@@ -24,7 +24,7 @@ GitHub Actions runs the default test suite via `.github/workflows/tests.yml`, us
 
 The default test suite also includes a focused local rule-execution smoke test. It runs the real `tenx2seuratrds`, `find_markers`, and `combine_markers` rule chain against `testdata/`, using Snakemake's `--use-conda` support and writing outputs under pytest's temporary directory. This catches broken R package imports, script argument drift, invalid Seurat object creation, and marker CSV schema changes without submitting to SLURM.
 
-The R output validator also checks that the Seurat object has at least 100 features and 100 cells; metadata rows match the cell count; barcode row names are present, unique, and nonempty; `orig.ident`, `nCount_RNA`, `nFeature_RNA`, `percent.mt`, and `seurat_clusters` metadata columns exist; RNA count and feature-count metadata values are finite and positive; mitochondrial percentages are finite and within `[0, 100]`; at least two clusters are present; PCA and UMAP reductions exist; the marker table is nonempty and has the expected columns; marker gene symbols are present and nonempty; marker numeric columns are finite; marker p-value and percent columns are within `[0, 1]`; marker clusters are present in the Seurat metadata; markers are reported for at least two clusters; and the marker `workflow` column matches the expected test workflow label. The test runner environment is defined in the repository-level `environment.yml`; the rule-specific R environment is still created by Snakemake from `workflow/envs/tenx2seuratrds.yml`. A separate lightweight checkpoint-expansion test uses a fake `Rscript` to materialize the `marker_manifest` checkpoint, verify that dynamic `find_markers` jobs are generated for each cluster id, and confirm that `combine_markers` receives the expected marker chunks.
+The R output validator also checks that the Seurat object has at least 100 features and 100 cells; metadata rows match the cell count; barcode row names are present, unique, and nonempty; `orig.ident`, `nCount_RNA`, `nFeature_RNA`, `percent.mt`, and `seurat_clusters` metadata columns exist; RNA count and feature-count metadata values are finite and positive; mitochondrial percentages are finite and within `[0, 100]`; at least two clusters are present; PCA and UMAP reductions exist; the marker table is nonempty and has the expected columns; marker gene symbols are present and nonempty; marker numeric columns are finite; marker p-value and percent columns are within `[0, 1]`; marker clusters are present in the Seurat metadata; markers are reported for at least two clusters; and the marker `workflow` column matches the expected test workflow label. The test runner environment is defined in the repository-level `environment.yml`; the rule-specific R environment is still created by Snakemake from `workflow/envs/tenx2seuratrds.yml`, using `workflow/envs/tenx2seuratrds.linux-64.pin.txt` on Linux to recreate the locked package set. A separate lightweight checkpoint-expansion test uses a fake `Rscript` to materialize the `marker_manifest` checkpoint, verify that dynamic `find_markers` jobs are generated for each cluster id, and confirm that `combine_markers` receives the expected marker chunks.
 
 
 ## Optional conda and container validation
@@ -37,7 +37,7 @@ pytest tests --run-container-validation
 pytest tests --run-doubletfinder-install
 ```
 
-`--run-conda-validation` creates each `workflow/envs/*.yml` environment in a pytest temporary directory and checks key R/Python package imports. To validate only one environment, pass `--conda-env-name ENV_FILE`, for example `--conda-env-name soupx.yml`; GitHub Actions uses this selector to run conda validation as one matrix job per env. `--run-container-validation` pulls the CellBender container with Docker, Apptainer, or Singularity. `--run-doubletfinder-install` runs the networked Snakemake `install_doubletfinder` rule and confirms that `DoubletFinder` can be imported from the created rule environment.
+`--run-conda-validation` creates each `workflow/envs/*.yml` environment in a pytest temporary directory and checks key R/Python package imports; on Linux it creates environments from the matching `*.linux-64.pin.txt` files so validation exercises the locked package specs used by Snakemake. To validate only one environment, pass `--conda-env-name ENV_FILE`, for example `--conda-env-name soupx.yml`; GitHub Actions uses this selector to run conda validation as one matrix job per env. `--run-container-validation` pulls the pinned CellBender container digest with Docker, Apptainer, or Singularity. `--run-doubletfinder-install` runs the networked Snakemake `install_doubletfinder` rule and confirms that `DoubletFinder` can be imported from the created rule environment.
 
 
 ## Optional full workflow run
@@ -50,12 +50,14 @@ pytest tests --run-workflow
 
 The full-run test calls `tests/run_test_workflow.sh`, which uses `testdata/samplesheet_test.tsv` and overrides the workflow output directory with `resultsDir=testdata/results`. The manifest in `tests/test_sample_rule_output_files.txt` is therefore written with paths under `testdata/results/`.
 
-For testing, omit `--snakemake-conda-prefix` so Snakemake uses its default `.snakemake/conda` location under the repository root. The runner assumes that the current environment already provides `snakemake` on `PATH`.
+For testing, omit `--snakemake-conda-prefix` so Snakemake uses its default `.snakemake/conda` location under the repository root. Snakemake includes the `*.linux-64.pin.txt` contents in environment hashes, so changing a lock file creates a new rule environment. The runner assumes that the current environment already provides `snakemake` on `PATH`.
+
+The GitHub-hosted full workflow action uses `tests/bin/cellbender` as a test shim for the CellBender rule because hosted runners do not provide the GPU-enabled CellBender container runtime used on the cluster. The shim copies the reference CellBender H5 outputs into the expected rule outputs, while the rest of the workflow runs normally and is still compared against the reference snapshot. Cluster/HPC runs of `pytest tests --run-workflow` do not use this shim unless `tests/bin` is explicitly added to `PATH`.
 
 
 ## Reference outputs
 
-The full workflow test compares regenerated files in `testdata/results/` against reference files under `tests/reference_outputs/`. The compared file list is in `tests/test_reference_output_files.txt`. Seurat `.rds` files are compared at the metadata-table level, marker CSVs are compared by columns and `(cluster, genesymbol)` rows with numeric tolerance, emptyDrops matrix files are compared after gzip decompression, CellBender H5 outputs are compared with `h5diff`, and remaining durable outputs are compared byte-for-byte.
+The full workflow test compares regenerated files in `testdata/results/` against reference files under `tests/reference_outputs/`. The compared file list is in `tests/test_reference_output_files.txt`. Seurat `.rds` files are compared at the metadata-table level, marker CSVs are compared by columns and `(cluster, genesymbol)` rows with numeric tolerance, emptyDrops matrix files are compared after gzip decompression, CellBender H5 outputs are compared with `h5diff`, and remaining durable outputs are compared byte-for-byte. Seurat metadata numeric columns use a strict default tolerance, except `neighborhood_purity`, which allows an absolute tolerance of `0.025` because it is a derived nearest-neighbor purity metric that can vary slightly across R/Bioconductor/platform builds even when barcode order and `seurat_clusters` match. Override that tolerance with `SEURAT_METADATA_NEIGHBORHOOD_PURITY_TOLERANCE` if needed.
 
 To refresh the reference snapshot after intentionally changing workflow behavior, first run the full test workflow so `testdata/results/` contains the desired outputs, then run:
 
diff --git a/tests/bin/cellbender b/tests/bin/cellbender
new file mode 100755
index 0000000..dc5f1da
--- /dev/null
+++ b/tests/bin/cellbender
@@ -0,0 +1,56 @@
+#!/usr/bin/env bash
+set -euo pipefail
+
+if [[ "${1:-}" != "remove-background" ]]; then
+    echo "test CellBender shim only supports: cellbender remove-background" >&2
+    exit 2
+fi
+shift
+
+if [[ "${1:-}" == "--help" ]]; then
+    echo "Usage: cellbender remove-background --input INPUT --output OUTPUT [--seed SEED] [--cuda]"
+    exit 0
+fi
+
+output=""
+while [[ $# -gt 0 ]]; do
+    case "$1" in
+        --output)
+            output="${2:-}"
+            shift 2
+            ;;
+        --input|--seed)
+            shift 2
+            ;;
+        --cuda)
+            shift
+            ;;
+        *)
+            shift
+            ;;
+    esac
+done
+
+if [[ -z "${output}" ]]; then
+    echo "test CellBender shim requires --output" >&2
+    exit 2
+fi
+
+source_dir="${TEST_WORKFLOW_FAKE_CELLBENDER_SOURCE:-}"
+if [[ -z "${source_dir}" ]]; then
+    echo "TEST_WORKFLOW_FAKE_CELLBENDER_SOURCE is required for the test CellBender shim" >&2
+    exit 127
+fi
+
+base_source="${source_dir}/cellbender_test.h5"
+filtered_source="${source_dir}/cellbender_test_filtered.h5"
+filtered_output="${output%.h5}_filtered.h5"
+
+if [[ ! -s "${base_source}" || ! -s "${filtered_source}" ]]; then
+    echo "test CellBender shim could not find reference H5 outputs under ${source_dir}" >&2
+    exit 1
+fi
+
+mkdir -p "$(dirname "${output}")"
+cp "${base_source}" "${output}"
+cp "${filtered_source}" "${filtered_output}"
diff --git a/tests/compare_seurat_metadata.R b/tests/compare_seurat_metadata.R
index 6a2e638..95f39ac 100644
--- a/tests/compare_seurat_metadata.R
+++ b/tests/compare_seurat_metadata.R
@@ -6,10 +6,19 @@ if (length(args) == 0 || length(args) %% 2 != 0) {
 suppressPackageStartupMessages(library("Seurat"))
 
 numeric_tolerance <- as.numeric(Sys.getenv("SEURAT_METADATA_NUMERIC_TOLERANCE", "1e-8"))
-if (is.na(numeric_tolerance) || length(numeric_tolerance) != 1) {
-  stop("SEURAT_METADATA_NUMERIC_TOLERANCE must be numeric", call. = FALSE)
+if (is.na(numeric_tolerance) || length(numeric_tolerance) != 1 || numeric_tolerance < 0) {
+  stop("SEURAT_METADATA_NUMERIC_TOLERANCE must be a non-negative number", call. = FALSE)
 }
 
+neighborhood_purity_tolerance <- as.numeric(Sys.getenv("SEURAT_METADATA_NEIGHBORHOOD_PURITY_TOLERANCE", "0.025"))
+if (is.na(neighborhood_purity_tolerance) || length(neighborhood_purity_tolerance) != 1 || neighborhood_purity_tolerance < 0) {
+  stop("SEURAT_METADATA_NEIGHBORHOOD_PURITY_TOLERANCE must be a non-negative number", call. = FALSE)
+}
+
+metadata_column_tolerances <- c(
+  neighborhood_purity = neighborhood_purity_tolerance
+)
+
 fail <- function(path, message) {
   stop(sprintf("%s: %s", path, message), call. = FALSE)
 }
@@ -30,14 +39,20 @@ compare_metadata_column <- function(current_path, column, current_values, refere
     if (any(comparable)) {
       diff <- abs(current_numeric[comparable] - reference_numeric[comparable])
       scale <- pmax(abs(current_numeric[comparable]), abs(reference_numeric[comparable]), 1)
-      bad <- diff > numeric_tolerance * scale
+      column_tolerance <- if (column %in% names(metadata_column_tolerances)) {
+        metadata_column_tolerances[[column]]
+      } else {
+        numeric_tolerance
+      }
+      bad <- diff > column_tolerance * scale
       if (any(bad)) {
         fail(
           current_path,
           sprintf(
-            "metadata column %s differs numerically; max abs diff %.12g",
+            "metadata column %s differs numerically; max abs diff %.12g exceeds tolerance %.12g",
             column,
-            max(diff)
+            max(diff),
+            column_tolerance
           )
         )
       }
diff --git a/tests/reference_outputs/testdata/results/cellbender/cellbender_test.h5 b/tests/reference_outputs/testdata/results/cellbender/cellbender_test.h5
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diff --git a/tests/reference_outputs/testdata/results/scdblfinder/seurat_scdblfinder_soupx_tenx_test.rds b/tests/reference_outputs/testdata/results/scdblfinder/seurat_scdblfinder_soupx_tenx_test.rds
index 56d02c4..5c5e72d 100644
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diff --git a/tests/reference_outputs/testdata/results/seurat_filtered/filtered_seurat_tenx_test.rds b/tests/reference_outputs/testdata/results/seurat_filtered/filtered_seurat_tenx_test.rds
index b7224f7..99d5516 100644
Binary files a/tests/reference_outputs/testdata/results/seurat_filtered/filtered_seurat_tenx_test.rds and b/tests/reference_outputs/testdata/results/seurat_filtered/filtered_seurat_tenx_test.rds differ
diff --git a/tests/reference_outputs/testdata/results/soupx/seurat_soupx_emptydrops_test.rds b/tests/reference_outputs/testdata/results/soupx/seurat_soupx_emptydrops_test.rds
index d3403c0..2de8ec6 100644
Binary files a/tests/reference_outputs/testdata/results/soupx/seurat_soupx_emptydrops_test.rds and b/tests/reference_outputs/testdata/results/soupx/seurat_soupx_emptydrops_test.rds differ
diff --git a/tests/reference_outputs/testdata/results/soupx/seurat_soupx_tenx_test.rds b/tests/reference_outputs/testdata/results/soupx/seurat_soupx_tenx_test.rds
index 848d99e..0d20fc7 100644
Binary files a/tests/reference_outputs/testdata/results/soupx/seurat_soupx_tenx_test.rds and b/tests/reference_outputs/testdata/results/soupx/seurat_soupx_tenx_test.rds differ
diff --git a/tests/run_test_workflow.sh b/tests/run_test_workflow.sh
index 316a5cc..60fcc22 100755
--- a/tests/run_test_workflow.sh
+++ b/tests/run_test_workflow.sh
@@ -13,6 +13,7 @@ set -euo pipefail
 usage() {
     echo "Usage: $0 [--conda-prefix PATH] [additional snakemake args...]"
     echo "Set TEST_WORKFLOW_SNAKEMAKE_ARGS to append whitespace-delimited Snakemake args before command-line extras."
+    echo "Set TEST_WORKFLOW_PROFILE_ARGS to replace the default Slurm/cannon profile args."
 }
 
 script_dir=$(cd "$(dirname "${BASH_SOURCE[0]}")" && pwd)
@@ -48,13 +49,38 @@ if ! command -v snakemake >/dev/null 2>&1; then
     exit 127
 fi
 
+workflow_seed=$(python - <<PYSEED
+from pathlib import Path
+import re
+
+config_text = Path("config/config.yaml").read_text()
+for line in config_text.splitlines():
+    match = re.match(r"^workflow_seed\s*:\s*([^#\s]+)", line)
+    if match:
+        print(int(match.group(1)))
+        break
+else:
+    raise SystemExit("workflow_seed is missing from config/config.yaml")
+PYSEED
+)
+export SCRNASEQ_PREPROCESS_SEED="${SCRNASEQ_PREPROCESS_SEED:-${workflow_seed}}"
+if [[ "${SCRNASEQ_PREPROCESS_SEED}" != "${workflow_seed}" ]]; then
+    echo "SCRNASEQ_PREPROCESS_SEED=${SCRNASEQ_PREPROCESS_SEED} does not match config workflow_seed=${workflow_seed}" >&2
+    exit 2
+fi
+
+profile_args=(--workflow-profile profiles/slurm --profile cannon)
+if [[ -n "${TEST_WORKFLOW_PROFILE_ARGS:-}" ]]; then
+    # Intended for simple Snakemake profile/executor args.
+    read -r -a profile_args <<< "${TEST_WORKFLOW_PROFILE_ARGS}"
+fi
+
 common_args=(
     --snakefile workflow/Snakefile
     --configfile config/config.yaml
     --config sampleTable=testdata/samplesheet_test.tsv resultsDir=testdata/results
     --use-conda
-    --workflow-profile profiles/slurm
-    --profile cannon
+    "${profile_args[@]}"
 )
 
 conda_prefix_args=()
@@ -62,6 +88,8 @@ if [[ -n "${snakemake_conda_prefix}" ]]; then
     conda_prefix_args=(--conda-prefix "${snakemake_conda_prefix}")
 fi
 
+test_workflow_jobs="${TEST_WORKFLOW_JOBS:-200}"
+
 env_snakemake_args=()
 if [[ -n "${TEST_WORKFLOW_SNAKEMAKE_ARGS:-}" ]]; then
     # Intended for simple Snakemake CLI tokens, such as --set-resources entries.
@@ -76,7 +104,7 @@ snakemake \
     "${conda_prefix_args[@]}" \
     --rerun-incomplete \
     --retries 2 \
-    --jobs 200 \
+    --jobs "${test_workflow_jobs}" \
     --latency-wait 600 \
     "${env_snakemake_args[@]}" \
     "${extra_snakemake_args[@]}"
diff --git a/tests/test_conda_container_validation.py b/tests/test_conda_container_validation.py
index f3893bb..09dda54 100644
--- a/tests/test_conda_container_validation.py
+++ b/tests/test_conda_container_validation.py
@@ -1,4 +1,5 @@
 import os
+import platform
 import re
 import shutil
 import subprocess
@@ -12,7 +13,6 @@
 RULE_DIR = Path("workflow/rules")
 TEST_SAMPLE_SHEET = Path("testdata/samplesheet_test.tsv")
 EXPECTED_ENV_FILES = {
-    "cellbender.yml",
     "doubletfinder.yml",
     "emptydrops.yml",
     "posthocfilter.yml",
@@ -22,9 +22,9 @@
 }
 CONDA_REFERENCE_RE = re.compile(r"conda:\s*\n\s*['\"]\.\./envs/([^'\"]+)['\"]")
 CONTAINER_RE = re.compile(r"container:\s*\n\s*['\"]([^'\"]+)['\"]")
+CELLBENDER_CONTAINER_URI = "docker://us.gcr.io/broad-dsde-methods/cellbender@sha256:093f2caf1ce4acae4541ea45e52ab7b220ca131ec73b4d1f664b85fe12850bae"
 
 R_IMPORTS_BY_ENV = {
-    "cellbender.yml": ["Seurat", "tidyverse", "bluster"],
     "doubletfinder.yml": ["Seurat", "tidyverse", "remotes", "fields", "Matrix", "KernSmooth", "ROCR", "igraph", "glmGamPoi", "bluster"],
     "emptydrops.yml": ["Seurat", "tidyverse", "DropletUtils", "scater", "glmGamPoi", "bluster", "Matrix", "R.utils"],
     "posthocfilter.yml": ["Seurat", "tidyverse", "glmGamPoi", "scater", "bluster"],
@@ -32,9 +32,7 @@
     "soupx.yml": ["Seurat", "tidyverse", "glmGamPoi", "bluster", "SoupX"],
     "tenx2seuratrds.yml": ["Seurat", "tidyverse", "scCustomize", "hdf5r", "glmGamPoi", "bluster", "presto"],
 }
-PYTHON_IMPORTS_BY_ENV = {
-    "cellbender.yml": ["cellbender"],
-}
+PYTHON_IMPORTS_BY_ENV = {}
 
 
 def repo_root():
@@ -73,6 +71,19 @@ def available_conda_frontend():
     raise AssertionError("neither mamba nor conda is available on PATH")
 
 
+def linux_conda_subdir():
+    if platform.system() == "Linux" and platform.machine() in {"x86_64", "AMD64"}:
+        return "linux-64"
+    return None
+
+
+def pin_file_for_env(env_path):
+    subdir = linux_conda_subdir()
+    if subdir is None:
+        return None
+    return env_path.with_suffix(f".{subdir}.pin.txt")
+
+
 def r_require_namespace_expr(packages):
     package_vector = ", ".join(repr(package) for package in packages)
     return (
@@ -100,6 +111,13 @@ def test_workflow_conda_env_files_are_well_formed_and_referenced_envs_exist():
         assert env.get("channel_priority") == "strict", env_path
         assert env.get("dependencies"), env_path
 
+        pin_path = pin_file_for_env(env_path)
+        if pin_path is not None:
+            assert pin_path.exists(), f"missing Snakemake conda pin file: {pin_path}"
+            pin_text = pin_path.read_text()
+            assert "@EXPLICIT" in pin_text, f"pin file is not an explicit conda spec: {pin_path}"
+            assert "conda-forge" in pin_text or "bioconda" in pin_text, pin_path
+
     referenced = set()
     for rule_path in sorted((root / RULE_DIR).glob("*.smk")):
         referenced.update(CONDA_REFERENCE_RE.findall(rule_path.read_text()))
@@ -116,7 +134,7 @@ def test_workflow_container_declarations_are_explicit_and_recognized():
             containers.append((rule_path, uri))
 
     assert containers, "no workflow container declarations found"
-    assert containers == [(root / "workflow/rules/cellbender.smk", "docker://us.gcr.io/broad-dsde-methods/cellbender:latest")]
+    assert containers == [(root / "workflow/rules/cellbender.smk", CELLBENDER_CONTAINER_URI)]
     for _, uri in containers:
         assert uri.startswith("docker://")
         assert ":" in uri.removeprefix("docker://"), f"container URI is missing a tag: {uri}"
@@ -135,8 +153,14 @@ def test_conda_env_solves_and_key_packages_import(tmp_path, pytestconfig, env_na
     prefix = tmp_path / env_name.removesuffix(".yml")
     conda = available_conda_frontend()
 
+    pin_path = pin_file_for_env(env_path)
+    if pin_path is not None and pin_path.exists():
+        create_cmd = [conda, "create", "--yes", "--prefix", str(prefix), "--file", str(pin_path)]
+    else:
+        create_cmd = [conda, "env", "create", "--yes", "--prefix", str(prefix), "--file", str(env_path)]
+
     create = run_command(
-        [conda, "env", "create", "--yes", "--prefix", str(prefix), "--file", str(env_path)],
+        create_cmd,
         root,
         timeout=1800,
     )
@@ -172,7 +196,7 @@ def test_cellbender_container_can_be_pulled(tmp_path, pytestconfig):
         pytest.skip("use --run-container-validation to pull workflow containers")
 
     root = repo_root()
-    uri = "docker://us.gcr.io/broad-dsde-methods/cellbender:latest"
+    uri = CELLBENDER_CONTAINER_URI
     docker_image = uri.removeprefix("docker://")
 
     if shutil.which("docker"):
diff --git a/workflow/envs/cellbender.yml b/workflow/envs/cellbender.yml
deleted file mode 100644
index da1aef2..0000000
--- a/workflow/envs/cellbender.yml
+++ /dev/null
@@ -1,15 +0,0 @@
-channels:
- - conda-forge
- - bioconda
-
-channel_priority: strict
-
-dependencies:
-  - r-tidyverse
-  - python=3.9  
-  - r-base
-  - r-seurat
-  - bioconductor-bluster
-  - pip          
-  - pip:       
-      - cellbender
diff --git a/workflow/envs/doubletfinder.linux-64.pin.txt b/workflow/envs/doubletfinder.linux-64.pin.txt
new file mode 100644
index 0000000..308bb41
--- /dev/null
+++ b/workflow/envs/doubletfinder.linux-64.pin.txt
@@ -0,0 +1,337 @@
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-64
+# created-by: conda 25.11.0
+@EXPLICIT
+https://conda.anaconda.org/conda-forge/noarch/_r-mutex-1.0.1-anacondar_1.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.5.20-hbd8a1cb_0.conda
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-h77eed37_3.conda
+https://conda.anaconda.org/conda-forge/noarch/kernel-headers_linux-64-4.18.0-he073ed8_9.conda
+https://conda.anaconda.org/conda-forge/noarch/libgcc-devel_linux-64-15.2.0-hcc6f6b0_119.conda
+https://conda.anaconda.org/conda-forge/linux-64/libgomp-15.2.0-he0feb66_19.conda
+https://conda.anaconda.org/conda-forge/noarch/libstdcxx-devel_linux-64-15.2.0-hd446a21_119.conda
+https://conda.anaconda.org/conda-forge/linux-64/libzlib-1.3.2-h25fd6f3_2.conda
+https://conda.anaconda.org/conda-forge/linux-64/pandoc-3.9.0.2-ha770c72_0.conda
+https://conda.anaconda.org/conda-forge/noarch/tzdata-2025c-hc9c84f9_1.conda
+https://conda.anaconda.org/conda-forge/linux-64/_openmp_mutex-4.5-20_gnu.conda
+https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
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diff --git a/workflow/envs/emptydrops.linux-64.pin.txt b/workflow/envs/emptydrops.linux-64.pin.txt
new file mode 100644
index 0000000..0eeef35
--- /dev/null
+++ b/workflow/envs/emptydrops.linux-64.pin.txt
@@ -0,0 +1,342 @@
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-64
+# created-by: conda 25.11.0
+@EXPLICIT
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diff --git a/workflow/envs/posthocfilter.linux-64.pin.txt b/workflow/envs/posthocfilter.linux-64.pin.txt
new file mode 100644
index 0000000..34c517a
--- /dev/null
+++ b/workflow/envs/posthocfilter.linux-64.pin.txt
@@ -0,0 +1,334 @@
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-64
+# created-by: conda 25.11.0
+@EXPLICIT
+https://conda.anaconda.org/conda-forge/noarch/_r-mutex-1.0.1-anacondar_1.tar.bz2
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+https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
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diff --git a/workflow/envs/scdblfinder.linux-64.pin.txt b/workflow/envs/scdblfinder.linux-64.pin.txt
new file mode 100644
index 0000000..015efe4
--- /dev/null
+++ b/workflow/envs/scdblfinder.linux-64.pin.txt
@@ -0,0 +1,357 @@
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-64
+# created-by: conda 25.11.0
+@EXPLICIT
+https://conda.anaconda.org/conda-forge/noarch/_r-mutex-1.0.1-anacondar_1.tar.bz2
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+https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-h77eed37_3.conda
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diff --git a/workflow/envs/soupx.linux-64.pin.txt b/workflow/envs/soupx.linux-64.pin.txt
new file mode 100644
index 0000000..c76e97b
--- /dev/null
+++ b/workflow/envs/soupx.linux-64.pin.txt
@@ -0,0 +1,322 @@
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-64
+# created-by: conda 25.11.0
+@EXPLICIT
+https://conda.anaconda.org/conda-forge/noarch/_r-mutex-1.0.1-anacondar_1.tar.bz2
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+https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
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diff --git a/workflow/envs/tenx2seuratrds.linux-64.pin.txt b/workflow/envs/tenx2seuratrds.linux-64.pin.txt
new file mode 100644
index 0000000..c7f1643
--- /dev/null
+++ b/workflow/envs/tenx2seuratrds.linux-64.pin.txt
@@ -0,0 +1,358 @@
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-64
+# created-by: conda 25.11.0
+@EXPLICIT
+https://conda.anaconda.org/conda-forge/noarch/_r-mutex-1.0.1-anacondar_1.tar.bz2
+https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.5.20-hbd8a1cb_0.conda
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+https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
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+https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-h77eed37_3.conda
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diff --git a/workflow/rules/cellbender.smk b/workflow/rules/cellbender.smk
index 5b9d8de..ca848d2 100644
--- a/workflow/rules/cellbender.smk
+++ b/workflow/rules/cellbender.smk
@@ -11,7 +11,7 @@ rule cellbender:
         workdir="scratch/cellbender/{sample}",
         seed=WORKFLOW_SEED
     container:
-        "docker://us.gcr.io/broad-dsde-methods/cellbender:latest"
+        "docker://us.gcr.io/broad-dsde-methods/cellbender@sha256:093f2caf1ce4acae4541ea45e52ab7b220ca131ec73b4d1f664b85fe12850bae"
     resources:
         mem_mb = lambda wildcards, attempt: int(50000 * (2 ** (attempt - 1))),
         slurm_partition = "gpu",
diff --git a/workflow/rules/install_doubletfinder.smk b/workflow/rules/install_doubletfinder.smk
index 4c5b95c..dc4bf7f 100644
--- a/workflow/rules/install_doubletfinder.smk
+++ b/workflow/rules/install_doubletfinder.smk
@@ -9,6 +9,6 @@ rule install_doubletfinder:
         "../envs/doubletfinder.yml"
     shell:
         """
-        Rscript -e "remotes::install_github('chris-mcginnis-ucsf/DoubletFinder', force = TRUE)" > {log} 2>&1
+        Rscript -e 'remotes::install_github("chris-mcginnis-ucsf/DoubletFinder", force = TRUE, dependencies = FALSE, upgrade = "never"); stopifnot(requireNamespace("DoubletFinder", quietly = TRUE), requireNamespace("Seurat", quietly = TRUE), requireNamespace("igraph", quietly = TRUE))' > {log} 2>&1
         touch {output}
         """