Run qPCR for PolyIC experiments

[AHuffmyer]

Aakriti, please help Valentina run qPCR from the PolyIC experiment that was run last week.

I have added Sam to this issue as well to help orient you to qPCR. Sam, could you involve Aakriti in any qPCR training/work coming up soon?

Aakriti, I'll connect you and Valentina over Slack as well.

[valevalenzuela]

Thanks! On Friday, I will run more qPCR to select the genes and made RNA extraction for other groups. I think Friday and next week, the help of Aakriti will be great

[AHuffmyer]

Great! @aakriti-vijay please follow up with Valentina regarding your schedule for Friday and next week.

[sr320]

@kubu4 can you bump this up?

[kubu4]

CITRATE SYNTHASE

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_15-32-08_Connect-citrate_synthase-01--Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_15-30-47_CFX96-citrate_synthase-02-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_16-31-12_Connect-citrate_synthase-03-Quantification-Cq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_15-32-08_Connect-citrate_synthase-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_15-30-47_CFX96-citrate_synthase-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_16-31-12_Connect-citrate_synthase-03.pdf

[kubu4]

DNMT1

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_13-34-02_Connect-DNMT1-01-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_13-29-34_CFX96-DNMT1-02%20Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_14-33-31_Connect-DNMT1-03-Quantification-Cq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_13-34-02_Connect-DNMT1-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_13-29-34_CFX96-DNMT1-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_14-33-31_Connect-DNMT1-03.pdf

[kubu4]

cGAS

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_11-25-14_Connect-cGAS-01-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_11-49-08_CFX96-cGAS-02-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/sam_2026-01-27_12-35-04_Connect-cGAS-03-QuantificationCq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_11-25-14_Connect-cGAS-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_11-49-08_CFX96-cGAS-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-27-qPCRs---M.gigas-Valentina-PolyIC-cGAS-citrate_synthase-and-DNMT1/report-sam_2026-01-27_12-35-04_Connect-cGAS-03.pdf

[kubu4]

ATP SYNTHASE

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_09-56-00_Connect-ATP_synthase-01-QuantificationCq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_10-13-15_CFX96-ATP_synthase-02-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_10-55-49_Connect-ATP_synthase-03-Quantification-Cq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_09-56-00_Connect-ATP_synthase-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_10-13-15_CFX96-ATP_synthase-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_10-55-49_Connect-ATP_synthase-03.pdf

[kubu4]

GAPDH

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_12-06-16_Connect-GAPDH-01-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_12-07-20_CFX96-GAPDH-02-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_13-05-59_Connect-GAPDH-03-Quantification-Cq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_12-06-16_Connect-GAPDH-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_12-07-20_CFX96-GAPDH-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_13-05-59_Connect-GAPDH-03.pdf

[kubu4]

HSP70

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_14-03-35_Connect-HSP70-01-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_14-25-31_CFX96-HSP70-02-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_15-00-57_Connect-HSP70-03-Quantification-Cq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_14-03-35_Connect-HSP70-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_14-25-31_CFX96-HSP70-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_15-00-57_Connect-HSP70-03.pdf

[kubu4]

HSP90

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28-16-06-43_Connect-HSP90-01-QuantificationCq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_16-07-47_CFX96-HSP90-02-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/sam_2026-01-28_17-07-09_Connect-HSP90-03-Quantification-Cq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28-16-06-43_Connect-HSP90-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_16-07-47_CFX96-HSP90-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-28-qPCRs---M.gigas-Valentina-PolyIC-ATP-synthase-GAPDH-HSP70-and-HSP90/report-sam_2026-01-28_17-07-09_Connect-HSP90-03.pdf

EDITED: Re-ordered qPCR reports URLs to be in numerical (plate) order.

[kubu4]

VIPERIN

NOTE: Ran out of cDNA for some samples, so not all samples were run in triplicate. Please see the report files for notes about which sample replicates (wells) were not run.

Cq data

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-29-qPCR---M.gigas-Valentina-PolyIC-VIPERIN/sam_2026-01-29_07-21-16_Connect-VIPERIN-01-QuantificationCq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-29-qPCR---M.gigas-Valentina-PolyIC-VIPERIN/sam_2026-01-29_07-39-06_CFX96-VIPERIN-02-Quantification-Cq_Results.csv

• https://raw.githubusercontent.com/RobertsLab/sams-notebook/refs/heads/master/posts/2026/2026-01-29-qPCR---M.gigas-Valentina-PolyIC-VIPERIN/sam_2026-01-29_08-24-59_Connect-VIPERIN-03-QuantificationCq_Results.csv

REPORTS

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-29-qPCR---M.gigas-Valentina-PolyIC-VIPERIN/report-sam_2026-01-29_07-21-16_Connect-VIPERIN-01.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-29-qPCR---M.gigas-Valentina-PolyIC-VIPERIN/report-sam_2026-01-29_07-39-06_CFX96-VIPERIN-02.pdf

• https://github.com/RobertsLab/sams-notebook/blob/master/posts/2026/2026-01-29-qPCR---M.gigas-Valentina-PolyIC-VIPERIN/report-sam_2026-01-29_08-24-59_Connect-VIPERIN-03.pdf

EDITED: Added note about replicates.

[kubu4]

Okay, that's currently all the primer sets I've run.

If we need to re-run any samples, we'll likely need to make additional cDNA.

If we want to run anymore primer sets, we'll definitely need to make additional cDNA.