Dear Cao,
We are trying to use the method in
"Cao J, Packer JS, Ramani V, Cusanovich DA, Huynh C, Daza R, Qiu X, Lee C, Furlan SN, Steemers FJ, Adey A, Waterston RH, Trapnell C, Shendure J. Comprehensive single-cell transcriptional profiling of a multicellular organism. Science. 2017 Aug 18;357(6352):661-667. doi: 10.1126/science.aam8940."
Is that possible to use the code here?
But somehow I found that you didn't use the Tn5 index.
Is that not necessary in this step?
Dear Cao,
We are trying to use the method in
"Cao J, Packer JS, Ramani V, Cusanovich DA, Huynh C, Daza R, Qiu X, Lee C, Furlan SN, Steemers FJ, Adey A, Waterston RH, Trapnell C, Shendure J. Comprehensive single-cell transcriptional profiling of a multicellular organism. Science. 2017 Aug 18;357(6352):661-667. doi: 10.1126/science.aam8940."
Is that possible to use the code here?
But somehow I found that you didn't use the Tn5 index.
Is that not necessary in this step?