diff --git a/src/cli/existing_mnemonic.rs b/src/cli/existing_mnemonic.rs
index 665d27b..a3ebae6 100644
--- a/src/cli/existing_mnemonic.rs
+++ b/src/cli/existing_mnemonic.rs
@@ -74,9 +74,22 @@ pub struct ExistingMnemonicSubcommandOpts {
     /// Deposit amount in ETH.
     /// For standard validators: exactly 32 ETH.
     /// For EIP-7251 compounding validators (0x02 withdrawal credentials): 32 to 2048 ETH.
-    #[arg(long, visible_alias = "deposit_amount", default_value = "32")]
+    /// Cannot be used together with --deposit-amount-gwei flag.
+    #[arg(
+        long,
+        visible_alias = "deposit_amount",
+        default_value = "32",
+        conflicts_with = "deposit_amount_gwei"
+    )]
     pub deposit_amount_eth: u64,
 
+    /// Deposit amount in Gwei.
+    /// For standard validators: exactly 32000000000 Gwei (32 ETH).
+    /// For EIP-7251 compounding validators (0x02 withdrawal credentials): 32000000000 to 2048000000000 Gwei.
+    /// Cannot be used together with --deposit-amount-eth flag.
+    #[arg(long, conflicts_with = "deposit_amount_eth")]
+    pub deposit_amount_gwei: Option<u64>,
+
     /// Use EIP-7251 compounding withdrawal credentials (0x02).
     ///
     /// When enabled, validators will use 0x02 withdrawal credentials which support
@@ -102,6 +115,12 @@ impl ExistingMnemonicSubcommandOpts {
             .clone()
             .map(|p| p.as_bytes().to_owned());
 
+        let deposit_amount_gwei = if let Some(gwei) = self.deposit_amount_gwei {
+            gwei
+        } else {
+            self.deposit_amount_eth * 1_000_000_000 // Convert ETH to Gwei
+        };
+
         let validators = Validators::new(
             Some(self.mnemonic.as_bytes()),
             password,
@@ -114,7 +133,7 @@ impl ExistingMnemonicSubcommandOpts {
             .export(
                 chain,
                 self.withdrawal_credentials.clone(),
-                self.deposit_amount_eth * 1_000_000_000, // Convert ETH to Gwei
+                deposit_amount_gwei,
                 self.compounding,
                 self.deposit_cli_version.clone(),
                 self.testnet_config.clone(),
diff --git a/src/cli/new_mnemonic.rs b/src/cli/new_mnemonic.rs
index e192406..bb15e62 100644
--- a/src/cli/new_mnemonic.rs
+++ b/src/cli/new_mnemonic.rs
@@ -63,9 +63,22 @@ pub struct NewMnemonicSubcommandOpts {
     /// Deposit amount in ETH.
     /// For standard validators: exactly 32 ETH.
     /// For EIP-7251 compounding validators (0x02 withdrawal credentials): 32 to 2048 ETH.
-    #[arg(long, visible_alias = "deposit_amount", default_value = "32")]
+    /// Cannot be used together with --deposit-amount-gwei flag.
+    #[arg(
+        long,
+        visible_alias = "deposit_amount",
+        default_value = "32",
+        conflicts_with = "deposit_amount_gwei"
+    )]
     pub deposit_amount_eth: u64,
 
+    /// Deposit amount in Gwei.
+    /// For standard validators: exactly 32000000000 Gwei (32 ETH).
+    /// For EIP-7251 compounding validators (0x02 withdrawal credentials): 32000000000 to 2048000000000 Gwei.
+    /// Cannot be used together with --deposit-amount-eth flag.
+    #[arg(long, conflicts_with = "deposit_amount_eth")]
+    pub deposit_amount_gwei: Option<u64>,
+
     /// Use EIP-7251 compounding withdrawal credentials (0x02).
     ///
     /// When enabled, validators will use 0x02 withdrawal credentials which support
@@ -91,6 +104,12 @@ impl NewMnemonicSubcommandOpts {
             .clone()
             .map(|p| p.as_bytes().to_owned());
 
+        let deposit_amount_gwei = if let Some(gwei) = self.deposit_amount_gwei {
+            gwei
+        } else {
+            self.deposit_amount_eth * 1_000_000_000 // Convert ETH to Gwei
+        };
+
         let validators = Validators::new(
             None,
             password,
@@ -103,7 +122,7 @@ impl NewMnemonicSubcommandOpts {
             .export(
                 chain,
                 self.withdrawal_credentials.clone(),
-                self.deposit_amount_eth * 1_000_000_000, // Convert ETH to Gwei
+                deposit_amount_gwei,
                 self.compounding,
                 self.deposit_cli_version.clone(),
                 self.testnet_config.clone(),
diff --git a/tests/e2e/new_mnemonic.rs b/tests/e2e/new_mnemonic.rs
index 3bffa5a..733c6d9 100644
--- a/tests/e2e/new_mnemonic.rs
+++ b/tests/e2e/new_mnemonic.rs
@@ -630,3 +630,79 @@ fn parse_kdf_function(keystore: &Keystore) -> String {
         .unwrap();
     kdf_function
 }
+
+/*
+    generate validator with deposit amount specified in gwei
+*/
+#[test]
+fn test_deposit_amount_gwei() -> Result<(), Box<dyn std::error::Error>> {
+    let chain = SupportedNetworks::Sepolia;
+    let decryption_password = "testtest";
+    let num_validators = "1";
+    let deposit_amount_gwei = "32000000000"; // 32 ETH in gwei
+
+    let mut cmd = Command::cargo_bin("eth-staking-smith")?;
+
+    cmd.arg("new-mnemonic");
+    cmd.arg("--chain");
+    cmd.arg(chain.to_string());
+    cmd.arg("--keystore_password");
+    cmd.arg(decryption_password);
+    cmd.arg("--num_validators");
+    cmd.arg(num_validators);
+    cmd.arg("--deposit-amount-gwei");
+    cmd.arg(deposit_amount_gwei);
+
+    cmd.assert().success();
+
+    let output = &cmd
+        .output()
+        .expect("could not get output from command")
+        .stdout;
+    let command_output = std::str::from_utf8(output).expect("could not parse output into string");
+    let generated_validator_json: ValidatorExports =
+        serde_json::from_str(command_output).expect("could not unmarshal command output");
+    let generated_deposit_data = generated_validator_json
+        .deposit_data
+        .get(0)
+        .expect("could not get generated deposit data")
+        .to_owned();
+
+    // verify the deposit amount is correct
+    assert_eq!(generated_deposit_data.amount, 32000000000);
+
+    generated_deposit_data
+        .validate(eth_staking_smith::chain_spec::chain_spec_for_network(&chain).unwrap());
+
+    Ok(())
+}
+
+/*
+    test that deposit-amount-eth and deposit-amount-gwei flags conflict
+*/
+#[test]
+fn test_conflicting_deposit_flags() -> Result<(), Box<dyn std::error::Error>> {
+    let chain = "sepolia";
+    let decryption_password = "testtest";
+    let num_validators = "1";
+
+    let mut cmd = Command::cargo_bin("eth-staking-smith")?;
+
+    cmd.arg("new-mnemonic");
+    cmd.arg("--chain");
+    cmd.arg(chain);
+    cmd.arg("--keystore_password");
+    cmd.arg(decryption_password);
+    cmd.arg("--num_validators");
+    cmd.arg(num_validators);
+    cmd.arg("--deposit-amount-eth");
+    cmd.arg("32");
+    cmd.arg("--deposit-amount-gwei");
+    cmd.arg("32000000000");
+
+    cmd.assert()
+        .failure()
+        .stderr(predicate::str::contains("cannot be used with"));
+
+    Ok(())
+}