diff --git a/src/PureHDF/VOL/Native/FileFormat/Level2/ObjectHeaderMessages/DataLayout/StoragePropertyDescriptions.cs b/src/PureHDF/VOL/Native/FileFormat/Level2/ObjectHeaderMessages/DataLayout/StoragePropertyDescriptions.cs
index 8e9b6c1d..58c13753 100644
--- a/src/PureHDF/VOL/Native/FileFormat/Level2/ObjectHeaderMessages/DataLayout/StoragePropertyDescriptions.cs
+++ b/src/PureHDF/VOL/Native/FileFormat/Level2/ObjectHeaderMessages/DataLayout/StoragePropertyDescriptions.cs
@@ -196,14 +196,16 @@ public static ChunkedStoragePropertyDescription4 Decode(NativeReadContext contex
 
     public override ushort GetEncodeSize()
     {
+        var encLen = ComputeEncodedLength(DimensionSizes);
+
         var encodeSize =
-            sizeof(byte) +
-            sizeof(byte) +
-            sizeof(byte) +
-            sizeof(ulong) * Rank +
-            sizeof(byte) +
+            sizeof(byte) +              // flags
+            sizeof(byte) +              // dimensionality (rank)
+            sizeof(byte) +              // dimension size encoded length
+            encLen * Rank +             // dimension sizes (variable byte width)
+            sizeof(byte) +              // chunk indexing type
             IndexingInformation.GetEncodeSize(Flags) +
-            sizeof(ulong);
+            sizeof(ulong);              // address
 
         return (ushort)encodeSize;
     }
@@ -218,17 +220,21 @@ public override void Encode(H5DriverBase driver)
         // dimensionality
         driver.Write(Rank);
 
-        // dimension size encoded length
-        driver.Write((byte)8);
-
-        // dimension sizes
-        for (int i = 0; i < Rank - 1; i++)
+        // dimension size encoded length: minimum number of bytes needed to encode
+        // the largest chunk dimension. libhdf5's H5D__chunk_set_sizes() in
+        // src/H5Dchunk.c strictly enforces (`!=` check) that this value matches its
+        // own calculation; hardcoding a different value (e.g. 8) produces files h5py /
+        // HDFView / MATLAB / Imaris reject with "stored chunk dimension encoding
+        // length does not match value calculated from chunk dimensions".
+        var encLen = ComputeEncodedLength(DimensionSizes);
+        driver.Write(encLen);
+
+        // dimension sizes (variable byte width per encLen, last entry is element size)
+        for (int i = 0; i < Rank; i++)
         {
-            driver.Write(DimensionSizes[i]);
+            WriteUtils.WriteUlongArbitrary(driver, DimensionSizes[i], encLen);
         }
 
-        driver.Write((ulong)4);
-
         // chunk indexing type
         var indexingType = IndexingInformation switch
         {
@@ -250,6 +256,33 @@ public override void Encode(H5DriverBase driver)
 
         IsDirty = false;
     }
+
+    // Mirrors libhdf5 H5D__chunk_set_sizes() byte-counting logic: counts how many
+    // 8-bit-shifted iterations bring the largest dimension value to zero. Returns 1
+    // even when all dims are zero (encoded length must be at least 1 per HDF5 spec).
+    private static byte ComputeEncodedLength(ulong[] dimensionSizes)
+    {
+        var maxValue = 0UL;
+
+        for (int i = 0; i < dimensionSizes.Length; i++)
+        {
+            if (dimensionSizes[i] > maxValue)
+                maxValue = dimensionSizes[i];
+        }
+
+        if (maxValue == 0)
+            return 1;
+
+        byte length = 0;
+
+        while (maxValue != 0)
+        {
+            length++;
+            maxValue >>= 8;
+        }
+
+        return length;
+    }
 }
 
 internal record class VirtualStoragePropertyDescription(
diff --git a/tests/PureHDF.Tests/Writing/DatasetTests@layout_chunked.cs b/tests/PureHDF.Tests/Writing/DatasetTests@layout_chunked.cs
index 8191696e..113db2bb 100644
--- a/tests/PureHDF.Tests/Writing/DatasetTests@layout_chunked.cs
+++ b/tests/PureHDF.Tests/Writing/DatasetTests@layout_chunked.cs
@@ -1,5 +1,6 @@
 using Xunit;
 using System.Reflection;
+using HDF.PInvoke;
 using PureHDF.Filters;
 
 namespace PureHDF.Tests.Writing;
@@ -386,4 +387,84 @@ public void ThrowsForInvalidChunkDimensions()
                 File.Delete(filePath);
         }
     }
+
+    // Cross-library compatibility test for chunk dimension encoded length.
+    // Pre-fix: chunked layouts always wrote (byte)8 as the encoded length, which
+    // libhdf5's H5D__chunk_set_sizes() rejects with
+    //   "stored chunk dimension encoding length does not match value calculated from chunk dimensions"
+    // because libhdf5 expects the *minimum* number of bytes needed to hold the
+    // largest chunk dimension. This test writes a chunked file through PureHDF
+    // and opens it through libhdf5 (via HDF.PInvoke); regression of the
+    // encoded-length bug surfaces as H5F.open returning a negative handle.
+    [Theory]
+    [InlineData(new uint[] { 10U })]                            // 1D, max 10 → 1 byte
+    [InlineData(new uint[] { 256U })]                           // 1D, max 256 → 2 bytes
+    [InlineData(new uint[] { 65536U })]                         // 1D, max 65536 → 3 bytes
+    [InlineData(new uint[] { 4U, 4U, 32U, 32U, 16U, 1U })]      // 6D real-world (microscopy)
+    public void ChunkedFile_IsReadableBy_libhdf5(uint[] chunkDims)
+    {
+        // Arrange — build N-D mock data matching the chunk shape (one chunk per dim)
+        var totalElements = 1;
+        foreach (var d in chunkDims)
+            totalElements *= (int)d;
+        var rawData = new int[totalElements];
+        for (var i = 0; i < totalElements; i++)
+            rawData[i] = i;
+
+        Array data;
+        if (chunkDims.Length == 1)
+        {
+            data = rawData;
+        }
+        else
+        {
+            var shape = new int[chunkDims.Length];
+            for (var i = 0; i < chunkDims.Length; i++)
+                shape[i] = (int)chunkDims[i];
+            var nd = Array.CreateInstance(typeof(int), shape);
+            Buffer.BlockCopy(rawData, 0, nd, 0, rawData.Length * sizeof(int));
+            data = nd;
+        }
+
+        var file = new H5File
+        {
+            ["chunked"] = new H5Dataset(data, chunks: chunkDims)
+        };
+
+        var filePath = Path.GetTempFileName();
+
+        // Act
+        file.Write(filePath);
+
+        // Assert — libhdf5 must accept the file (negative handle = error)
+        try
+        {
+            var fileId = H5F.open(filePath, H5F.ACC_RDONLY);
+            try
+            {
+                Assert.True(fileId >= 0, $"H5F.open rejected PureHDF chunked file (handle={fileId})");
+
+                var datasetId = H5D.open(fileId, "chunked");
+                try
+                {
+                    Assert.True(datasetId >= 0, $"H5D.open rejected chunked dataset (handle={datasetId})");
+                }
+                finally
+                {
+                    if (datasetId >= 0)
+                        _ = H5D.close(datasetId);
+                }
+            }
+            finally
+            {
+                if (fileId >= 0)
+                    _ = H5F.close(fileId);
+            }
+        }
+        finally
+        {
+            if (File.Exists(filePath))
+                File.Delete(filePath);
+        }
+    }
 }
\ No newline at end of file